Evaluating the potential role of nitric oxide as a mediator of hydrostatic edema mediated intestinal contractile dysfunction.
J Surg Res
; 163(1): 102-9, 2010 Sep.
Article
en En
| MEDLINE
| ID: mdl-20605598
ABSTRACT
BACKGROUND:
Administration of L-nil, a selective inhibitor of inducible nitric oxide synthase (iNOS), improves ileus in an animal model of resuscitation induced intestinal edema. The purpose of this study was to elucidate the iNOS/nitric oxide (NO) signal transduction pathway in intestinal edema. MATERIALS ANDMETHODS:
Male Sprague Dawley rats were divided into two groups; CONTROL and RESUS+VH (edema, 80 cc/kg normal saline (resuscitation) with mesenteric venous hypertension). iNOS mRNA and protein, iNOS activity, NO tissue levels, soluble guanylyl cyclase (sGC) expression, and cyclic guanosine monophosphate (cGMP) levels were measured. As a functional endpoint, we evaluated intestinal contractile strength and frequency in L-nil treated animals.RESULTS:
Edema was associated with increased iNOS mRNA and protein expression without subsequent increases in iNOS activity or tissue NO levels. There was no significant change in sGC expression or increase in cGMP induced by edema. Administration of L-nil did not decrease edema development or preserve contractile strength, but increased contractile frequency.CONCLUSION:
Hydrostatic intestinal edema is not associated with increased iNOS activity or tissue NO levels. Administration of L-nil in edema increases intestinal contractile frequency. This may represent a potential mechanism for the amelioration of ileus seen with the administration of L-nil.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
GMP Cíclico
/
Edema
/
Óxido Nítrico Sintasa de Tipo II
/
Motilidad Gastrointestinal
/
Enfermedades Intestinales
/
Óxido Nítrico
Límite:
Animals
Idioma:
En
Revista:
J Surg Res
Año:
2010
Tipo del documento:
Article
País de afiliación:
Estados Unidos