A novel two-component Tobacco mosaic virus-based vector system for high-level expression of multiple therapeutic proteins including a human monoclonal antibody in plants.
Virology
; 405(1): 93-9, 2010 Sep 15.
Article
en En
| MEDLINE
| ID: mdl-20673747
ABSTRACT
Expression of multiple therapeutic proteins from Tobacco mosaic virus (TMV)-based vectors was not successful when plants were coinoculated with a mixture of two TMV vectors engineered to express two foreign genes individually. Here, we have engineered and developed a defective RNA (dRNA)-based TMV vector (dRT-V) that utilizes two components of the same virus, with the dRNA component depending on the helper virus for replication. Agrobacterium-mediated coinoculation of Nicotiana benthamiana plants with both components of the dRT-V resulted in high-level expression of a human growth hormone and a lichenase-fused lethal factor protein of Bacillus anthracis. Furthermore, both heavy and light chains were expressed and assembled into a monoclonal antibody (mAb) specific to the protective antigen of B. anthracis, and the average yield of the purified antibody obtained was 120 mg/kg of fresh tissue. Our data suggest that dRT-V has a potential for rapid, cost-effective, large-scale manufacturing of multiple therapeutic proteins including mAbs in response to any biological emergencies.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Nicotiana
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Virus del Mosaico del Tabaco
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Regulación de la Expresión Génica
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Vectores Genéticos
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Anticuerpos Antibacterianos
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Anticuerpos Monoclonales
Límite:
Humans
Idioma:
En
Revista:
Virology
Año:
2010
Tipo del documento:
Article
País de afiliación:
Estados Unidos