Arabidopsis DREB2C functions as a transcriptional activator of HsfA3 during the heat stress response.
Biochem Biophys Res Commun
; 401(2): 238-44, 2010 Oct 15.
Article
en En
| MEDLINE
| ID: mdl-20849812
ABSTRACT
The dehydration-responsive element binding protein (DREB) family is important in regulating plant responses to abiotic stresses. DREB2C is one of the Arabidopsis class 2 DREBs and is induced by heat stress (HS). Here, we present data concerning the interaction of DREB2C with heat shock factor A3 (HsfA3) in the HS signal transduction cascade. RT-PCR showed that HsfA3 is the most up-regulated gene among the 21 Arabidopsis Hsfs in transgenic plants over-expressing DREB2C. DREB2C and HsfA3 displayed similar transcription patterns in response to HS and DREB2C specifically transactivated the DRE-dependent transcription of HsfA3 in Arabidopsis mesophyll protoplasts. Yeast one-hybrid assays and invitro electrophoretic mobility shift assays further showed that DREB2C interacts with two DREs located in the HsfA3 promoter with a binding preference for the distal DRE2. Deletion mutants of DREB2C indicated that transactivation activity was located in the C-terminal region. In addition, dual activator-reporter assays showed that the induction of heat shock protein (Hsp) genes in transgenic plants could be attributed to the transcriptional activity of HsfA3. Taken together, these results indicate that DREB2C and HsfA3 are key players in regulating the heat tolerance of Arabidopsis.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Proteínas de Plantas
/
Factores de Transcripción
/
Activación Transcripcional
/
Transactivadores
/
Arabidopsis
/
Regulación de la Expresión Génica de las Plantas
/
Respuesta al Choque Térmico
/
Proteínas de Arabidopsis
/
Proteínas de Unión al ADN
/
Proteínas de Choque Térmico
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2010
Tipo del documento:
Article