Transcriptome analysis indicates an enhanced activation of adaptive and innate immunity by chlamydia-infected murine epithelial cells treated with interferon γ.
J Infect Dis
; 202(9): 1405-14, 2010 Nov 01.
Article
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| MEDLINE
| ID: mdl-20868270
ABSTRACT
BACKGROUND:
Interferon γ (IFNγ) is the major cytokine involved in the elimination of Chlamydia infection. Despite its importance, the combined effect of Chlamydia infection and IFNγ on the gene expression of murine epithelial cells has only partially been described.METHODS:
The DNA chip method was used to evaluate the impact of IFNγ and both the human strain Chlamydia trachomatis L2 infection and the murine strain Chlamydia muridarum infection on the transcriptome of murine epithelial cells.RESULTS:
The gene expression analysis revealed that IFNγ had an enhancing effect on both the upregulation and downregulation of the epithelial gene expression. The influenced gene functional classes included cytokine and chemokine expression, antigen presentation, apoptosis, and genes involved in basic metabolic processes such as fatty acid oxidation. We also detected the upregulation of various genes that could be directly antichlamydial, such as members of the p47 GTPase family, inducible nitric oxide synthase, and monokine induced by IFNγ (MIG). As a functional validation of DNA chip data, we measured the antichlamydial effect of MIG on the extracellular form of Chlamydia.CONCLUSIONS:
Our results show that IFNγ is a key cytokine that primes epithelial cells to activate adaptive and innate immunity and to express antichlamydial effector genes both intracellularly and extracellularly.
Texto completo:
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Bases de datos:
MEDLINE
Asunto principal:
Chlamydia trachomatis
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Interferones
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Perfilación de la Expresión Génica
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Chlamydia muridarum
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Células Epiteliales
Límite:
Animals
Idioma:
En
Revista:
J Infect Dis
Año:
2010
Tipo del documento:
Article
País de afiliación:
Hungria