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Identification of streptococcal proteins reacting with sera from Behçet's disease and rheumatic disorders.
Cho, Sung Bin; Lee, Ju Hee; Ahn, Keun Jae; Cho, Suhyun; Park, Yong-Beom; Lee, Soo-Kon; Bang, Dongsik; Lee, Kwang Hoon.
Afiliación
  • Cho SB; Department of Dermatology, Yonsei University College of Medicine, Seoul, Korea. sbcho@yuhs.ac
Clin Exp Rheumatol ; 28(4 Suppl 60): S31-8, 2010.
Article en En | MEDLINE | ID: mdl-20868568
ABSTRACT

OBJECTIVES:

We evaluated the reactivity of sera from Behçet's disease (BD), systemic lupus erythematosus (SLE), dermatomyositis (DM), rheumatoid arthritis (RA), and Takayasu's arteritis (TA) patients against human α-enolase and streptococcal α-enolase, and identified additional streptococcal antigens.

METHODS:

Enzyme-linked immunosorbent assay (ELISA) and immunoblotting were performed using sera from patients with BD, SLE, DM, RA, and TA and healthy volunteers (control) against human α-enolase and streptococcal α-enolase. Immunoblot analysis and matrix-assisted laser desorption ionisation-time-of-flight mass spectrometry were used to identify and recombine other streptococcal antigens.

RESULTS:

Specific positive signals against recombinant human α-enolase were detected by IgM ELISA of serum samples from 50% of BD, 14.3% of SLE, 57.1% of DM, 42.9% of RA, and 57.1% of TA patients. Specific positive signals against streptococcal α-enolase were detected from 42.9% of BD, 14.3% of DM, and 14.3% of TA patients. No SLE and RA sera reacted against streptococcal α-enolase antigen. Streptococcal proteins reacting with sera were identified as hypothetical protein (HP) for SLE and DM patients, acid phosphatase (AP) for RA patients, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) for TA patients.

CONCLUSIONS:

We observed that RA patients did not present serum reactivity against either HP or GAPDH though BD, SLE, DM, and TA patients did. Also, AP reacted with sera from BD, SLE, DM, RA, and TA patients.
Asunto(s)
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Bases de datos: MEDLINE Asunto principal: Artritis Reumatoide / Streptococcus sanguis / Proteínas Bacterianas / Síndrome de Behçet / Dermatomiositis / Lupus Eritematoso Sistémico / Anticuerpos Antibacterianos Tipo de estudio: Diagnostic_studies / Observational_studies Límite: Humans Idioma: En Revista: Clin Exp Rheumatol Año: 2010 Tipo del documento: Article
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Bases de datos: MEDLINE Asunto principal: Artritis Reumatoide / Streptococcus sanguis / Proteínas Bacterianas / Síndrome de Behçet / Dermatomiositis / Lupus Eritematoso Sistémico / Anticuerpos Antibacterianos Tipo de estudio: Diagnostic_studies / Observational_studies Límite: Humans Idioma: En Revista: Clin Exp Rheumatol Año: 2010 Tipo del documento: Article