cis-Regulatory remodeling of the SCL locus during vertebrate evolution.
Mol Cell Biol
; 30(24): 5741-51, 2010 Dec.
Article
en En
| MEDLINE
| ID: mdl-20956563
ABSTRACT
Development progresses through a sequence of cellular identities which are determined by the activities of networks of transcription factor genes. Alterations in cis-regulatory elements of these genes play a major role in evolutionary change, but little is known about the mechanisms responsible for maintaining conserved patterns of gene expression. We have studied the evolution of cis-regulatory mechanisms controlling the SCL gene, which encodes a key transcriptional regulator of blood, vasculature, and brain development and exhibits conserved function and pattern of expression throughout vertebrate evolution. SCL cis-regulatory elements are conserved between frog and chicken but accrued alterations at an accelerated rate between 310 and 200 million years ago, with subsequent fixation of a new cis-regulatory pattern at the beginning of the mammalian radiation. As a consequence, orthologous elements shared by mammals and lower vertebrates exhibit functional differences and binding site turnover between widely separated cis-regulatory modules. However, the net effect of these alterations is constancy of overall regulatory inputs and of expression pattern. Our data demonstrate remarkable cis-regulatory remodelling across the SCL locus and indicate that stable patterns of expression can mask extensive regulatory change. These insights illuminate our understanding of vertebrate evolution.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Factores de Transcripción
/
Vertebrados
/
Secuencias Reguladoras de Ácidos Nucleicos
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Proteínas Proto-Oncogénicas
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Regulación del Desarrollo de la Expresión Génica
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Proteínas de Xenopus
/
Proteínas de Unión al ADN
/
Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico
/
Evolución Biológica
Tipo de estudio:
Clinical_trials
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Mol Cell Biol
Año:
2010
Tipo del documento:
Article
País de afiliación:
Reino Unido