Inhibition of CYP3A mRNA and protein expression, and enzymatic activity, by enrofloxacin in chickens.
J Vet Pharmacol Ther
; 33(6): 546-50, 2010 Dec.
Article
en En
| MEDLINE
| ID: mdl-21062306
ABSTRACT
This study was to investigate the effect of enrofloxacin (EF) on CYP3A in chicken by using quantitative reverse transcription-polymerase chain reaction and immunodetected. The treated chickens were given 5, 25 and 125 mg/kg of EF while the control chickens were treated with the same volume saline. There was no significant difference between the low dose group and controls in the concentration of hepatic microsome protein and total CYP content, while the middle and high dose EF caused the down regulation. Depression of the CYP3A activity, mRNA and protein were observed in treated chickens, and the inhibition degree was different from each group. It was concluded that EF caused the inhibition of CYP3A both in genetic transcription and protein levels. But the inhibition metabolism still needs further researches.
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Bases de datos:
MEDLINE
Asunto principal:
Biosíntesis de Proteínas
/
Fluoroquinolonas
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Citocromo P-450 CYP3A
/
Antiinfecciosos
Límite:
Animals
Idioma:
En
Revista:
J Vet Pharmacol Ther
Año:
2010
Tipo del documento:
Article
País de afiliación:
China