Multiplexed analysis of circulating cell-free fetal nucleic acids for noninvasive prenatal diagnostic RHD testing.
Am J Obstet Gynecol
; 204(3): 251.e1-6, 2011 Mar.
Article
en En
| MEDLINE
| ID: mdl-21092930
ABSTRACT
OBJECTIVE:
The objective of the study was the evaluation of a novel multiplex assay to detect fetal Rh blood group D-antigen gene (RHD) loci in maternal plasma from RhD-negative, pregnant women. STUDYDESIGN:
An RHD genotyping assay was designed to detect exons 4, 5, 7, and 10 and RHDΨ (pseudogene) of the RHD gene along with a Y chromosome-specific assay and a generic polymerase chain reaction amplification control. Plasma samples from 150 RhD-negative pregnant women were assayed for fetal RHD genotype using the MassARRAY system.RESULTS:
The fetal RHD status of 148 of 150 samples (98.7%) was correctly classified; 86 (57.3%) and 62 (41.3%) were positive and negative, respectively.CONCLUSION:
This study demonstrates that noninvasive prenatal diagnostics with a single-reaction multiplexed assay is a viable path toward routine characterization of fetal RHD genotypes using circulating cell-free fetal DNA in maternal plasma on the MassARRAY system and is perhaps preferable to serologic testing as currently used clinically.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Sistema del Grupo Sanguíneo Rh-Hr
/
ADN
Tipo de estudio:
Diagnostic_studies
Límite:
Adolescent
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Adult
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Female
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Humans
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Pregnancy
Idioma:
En
Revista:
Am J Obstet Gynecol
Año:
2011
Tipo del documento:
Article
País de afiliación:
Estados Unidos