Next-generation sequencing of Coccidioides immitis isolated during cluster investigation.
Emerg Infect Dis
; 17(2): 227-32, 2011 Feb.
Article
en En
| MEDLINE
| ID: mdl-21291593
ABSTRACT
Next-generation sequencing enables use of whole-genome sequence typing (WGST) as a viable and discriminatory tool for genotyping and molecular epidemiologic analysis. We used WGST to confirm the linkage of a cluster of Coccidioides immitis isolates from 3 patients who received organ transplants from a single donor who later had positive test results for coccidioidomycosis. Isolates from the 3 patients were nearly genetically identical (a total of 3 single-nucleotide polymorphisms identified among them), thereby demonstrating direct descent of the 3 isolates from an original isolate. We used WGST to demonstrate the genotypic relatedness of C. immitis isolates that were also epidemiologically linked. Thus, WGST offers unique benefits to public health for investigation of clusters considered to be linked to a single source.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Trasplante de Órganos
/
Técnicas de Tipificación Micológica
/
Genoma Fúngico
/
Análisis de Secuencia de ADN
/
Coccidioides
/
Coccidioidomicosis
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
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Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Emerg Infect Dis
Asunto de la revista:
DOENCAS TRANSMISSIVEIS
Año:
2011
Tipo del documento:
Article
País de afiliación:
Estados Unidos