Distribution of HPV genotypes in women with cervical cancer in Auckland, New Zealand; a review of 50 specimens between 2000-2006.
Aust N Z J Obstet Gynaecol
; 51(1): 67-70, 2011 Feb.
Article
en En
| MEDLINE
| ID: mdl-21299512
ABSTRACT
BACKGROUND:
In New Zealand, around two hundred women are diagnosed with cervical cancer annually, with approximately seventy deaths from cervical cancer per year.AIM:
Our aim was to determine the distribution of oncogenic HPV genotypes in biopsy specimens from women with diagnosed cervical cancers in the Auckland region of New Zealand between 2000-2006. MATERIALS ANDMETHODS:
Confirmed cases of cervical carcinoma were identified from the local pathology register, and representative tissue samples were taken from these blocks. Sections were deparaffinised, and DNA was extracted according to standard protocols. Samples were subject to PCR amplification using L1 consensus primer sets MY09/11 and GP5/6. Further type-specific amplification was performed on positive samples, using an in-house primer sequence based on target sequences within the E6 gene. Remaining samples were typed by a Linear Array Assay, or by DNA sequencing.RESULTS:
HPV DNA was detected in 100% of cases. In 49/50 samples, the HPV genotype was identified, with a total of 14 different HPV genotypes detectable. Together HPV-16 and 18 were found in 41/49 cases (83.6%) either singly or in combination.DISCUSSION:
Our findings suggest that the distribution of HPV genotypes in New Zealand is similar to that of other geographic areas.CONCLUSION:
Ongoing surveillance is warranted to ensure appropriate genotype selection for prophylactic HPV vaccinations.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Carcinoma de Células Escamosas
/
Neoplasias del Cuello Uterino
/
Infecciones por Papillomavirus
/
Papillomavirus Humano 16
/
Papillomavirus Humano 18
Tipo de estudio:
Guideline
/
Prognostic_studies
Límite:
Female
/
Humans
País/Región como asunto:
Oceania
Idioma:
En
Revista:
Aust N Z J Obstet Gynaecol
Año:
2011
Tipo del documento:
Article
País de afiliación:
Nueva Zelanda