Transcription of reference genes used for quantitative RT-PCR in Atlantic salmon is affected by viral infection.
Vet Res
; 42: 8, 2011 Jan 18.
Article
en En
| MEDLINE
| ID: mdl-21314970
ABSTRACT
Relative quantification using RT-qPCR is a widely used method for transcription profiling. Transcript levels of target genes in fish after experimental infection is often reported without documentation of stably transcribed reference genes. We present results demonstrating that transcription of typically used reference genes in Atlantic salmon is not stable during experimental infection with salmon pancreas disease virus (SPDV). Transcript levels 0 to 6 weeks after challenge revealed statistically significant changes between time-points that corresponded with a peak in viral load 3 weeks after challenge. The results emphasize the need for thorough method validation prior to transcriptional studies during viral infections.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Infecciones por Alphavirus
/
Alphavirus
/
Salmo salar
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Perfilación de la Expresión Génica
/
Enfermedades de los Peces
Tipo de estudio:
Evaluation_studies
Límite:
Animals
Idioma:
En
Revista:
Vet Res
Asunto de la revista:
MEDICINA VETERINARIA
Año:
2011
Tipo del documento:
Article
País de afiliación:
Noruega