A multiplex polymerase chain reaction method for rapid pneumococcal serotype determination in childhood empyema.
Diagn Microbiol Infect Dis
; 69(3): 245-9, 2011 Mar.
Article
en En
| MEDLINE
| ID: mdl-21353946
ABSTRACT
Nonvaccine serotypes are increasingly responsible for pneumococcal empyema. We developed a one-step polymerase chain reaction for identifying all serotypes covered by the 13-valent conjugate vaccine. Over 1 year, we identified serotypes 19A (n = 6), 1 (n = 2), 3 (n = 1) and 7F/A (n = 2), both in culture-positive and culture-negative pleural fluid from 11 children with empyema.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Infecciones Neumocócicas
/
Streptococcus pneumoniae
/
Serotipificación
/
Reacción en Cadena de la Polimerasa
/
Empiema Pleural
Límite:
Adolescent
/
Child
/
Child, preschool
/
Female
/
Humans
/
Infant
/
Male
Idioma:
En
Revista:
Diagn Microbiol Infect Dis
Año:
2011
Tipo del documento:
Article
País de afiliación:
Francia