A novel rice calmodulin-like gene, OsMSR2, enhances drought and salt tolerance and increases ABA sensitivity in Arabidopsis.
Planta
; 234(1): 47-59, 2011 Jul.
Article
en En
| MEDLINE
| ID: mdl-21359958
ABSTRACT
Many abiotic stimuli, such as drought and salt stresses, elicit changes in intracellular calcium levels that serve to convey information and activate adaptive responses. Ca²âº signals are perceived by different Ca²âº sensors, and calmodulin (CaM) is one of the best-characterized Ca²âº sensors in eukaryotes. Calmodulin-like (CML) proteins also exist in plants, but their functions at the physiological and molecular levels are largely unknown. In this report, we present data on OsMSR2 (Oryza sativa L. Multi-Stress-Responsive gene 2), a novel calmodulin-like protein gene isolated from rice Pei'ai 64S (Oryza sativa L.). Expression of OsMSR2 was strongly up-regulated by a wide spectrum of stresses, including cold, drought, and heat in different tissues at different developmental stages of rice, as revealed by both microarray and quantitative real-time RT-PCR analyses. Analysis of the recombinant OsMSR2 protein demonstrated its potential ability to bind Ca²âº in vitro. Expression of OsMSR2 conferred enhanced tolerance to high salt and drought in Arabidopsis (Arabidopsis thaliana) accompanied by altered expression of stress/ABA-responsive genes. Transgenic plants also exhibited hypersensitivity to ABA during the seed germination and post-germination stages. The results suggest that expression of OsMSR2 modulated salt and drought tolerance in Arabidopsis through ABA-mediated pathways.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Oryza
/
Calmodulina
/
Arabidopsis
/
Deshidratación
/
Tolerancia a la Sal
/
Sequías
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Planta
Año:
2011
Tipo del documento:
Article
País de afiliación:
China