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Quantitative proteomic analysis of dystrophic dog muscle.
Guevel, Laetitia; Lavoie, Jessie R; Perez-Iratxeta, Carolina; Rouger, Karl; Dubreil, Laurence; Feron, Marie; Talon, Sophie; Brand, Marjorie; Megeney, Lynn A.
Afiliación
  • Guevel L; CNRS UMR6204, Faculté des Sciences et des Techniques, F-44322 Nantes Cedex 3, France. laetitia.guevel@univ-nantes.fr
J Proteome Res ; 10(5): 2465-78, 2011 May 06.
Article en En | MEDLINE | ID: mdl-21410286
ABSTRACT
Duchenne muscular dystrophy (DMD) is caused by null mutations in the dystrophin gene, leading to progressive and unrelenting muscle loss. Although the genetic basis of DMD is well resolved, the cellular mechanisms associated with the physiopathology remain largely unknown. Increasing evidence suggests that secondary mechanisms, as the alteration of key signaling pathways, may play an important role. In order to identify reliable biomarkers and potential therapeutic targets, and taking advantage of the clinically relevant Golden Retriever Muscular Dystrophy (GRMD) dog model, a proteomic study was performed. Isotope-coded affinity tag (ICAT) profiling was used to compile quantitative changes in protein expression profiles of the vastus lateralis muscles of 4-month old GRMD vs healthy dogs. Interestingly, the set of under-expressed proteins detected appeared primarily composed of metabolic proteins, many of which have been shown to be regulated by the transcriptional peroxisome proliferator-activated receptor-gamma co-activator 1 alpha (PGC-1α). Subsequently, we were able to showed that PGC1-α expression is dramatically reduced in GRMD compared to healthy muscle. Collectively, these results provide novel insights into the molecular pathology of the clinically relevant animal model of DMD, and indicate that defective energy metabolism is a central hallmark of the disease in the canine model.
Asunto(s)

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Biomarcadores / Músculo Esquelético / Distrofia Muscular de Duchenne / Proteoma / Proteómica / Metabolismo Energético Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Proteome Res Asunto de la revista: BIOQUIMICA Año: 2011 Tipo del documento: Article País de afiliación: Francia

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Biomarcadores / Músculo Esquelético / Distrofia Muscular de Duchenne / Proteoma / Proteómica / Metabolismo Energético Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Proteome Res Asunto de la revista: BIOQUIMICA Año: 2011 Tipo del documento: Article País de afiliación: Francia