Myelomonocytic THP-1 cells for in vitro testing of immunomodulatory properties of nanoparticles.
J Biomed Nanotechnol
; 7(1): 209-10, 2011 Feb.
Article
en En
| MEDLINE
| ID: mdl-21485874
ABSTRACT
The use of nanoparticles for new therapeutic and diagnostics options represents a new risk for individuals exposed to such compounds. The myelomonocytic cell line THP-1 could be a useful alternative to human peripheral blood mononuclear cells (PBMC) to test for effects of drugs and compounds. Stimulation degree of cells can be monitored by measurement of neopterin and/or the kynurenine to tryptophan ratio. The method is robust and reproducible in the range of 0.1-1.0 microg/ml of LPS. However, compared to the PBMC assay it will not reveal any effect on the T-cell interaction.
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Bases de datos:
MEDLINE
Asunto principal:
Leucemia Mieloide
/
Lipopolisacáridos
/
Nanopartículas
/
Inmunomodulación
/
Inmunidad Innata
Límite:
Humans
Idioma:
En
Revista:
J Biomed Nanotechnol
Año:
2011
Tipo del documento:
Article
País de afiliación:
Austria