Characterization of and host response to tyramine substituted-hyaluronan enriched fascia extracellular matrix.
J Mater Sci Mater Med
; 22(6): 1465-77, 2011 Jun.
Article
en En
| MEDLINE
| ID: mdl-21553156
ABSTRACT
Naturally-occurring biomaterial scaffolds derived from extracellular matrix (ECM) have been previously investigated for soft tissue repair. We propose to enrich fascia ECM with high molecular weight tyramine substituted-hyaluronan (TS-HA) to modulate inflammation associated with implantation and enhance fibroblast infiltration. As critical determinants of constructive remodeling, the host inflammatory response and macrophage polarization to TS-HA enriched fascia were characterized in a rat abdominal wall model. TS-HA treated fascia with cross-linking had a similar lymphocyte (P = 0.11) and plasma cell (P = 0.13) densities, greater macrophage (P = 0.001) and giant cell (P < 0.0001) densities, and a lower density of fibroblast-like cells (P < 0.0001) than water treated controls. Treated fascia, with or without cross-linking, exhibited a predominantly M2 pro-remodeling macrophage profile similar to water controls (P = 0.82), which is suggestive of constructive tissue remodeling. Our findings demonstrated that HA augmentation can alter the host response to an ECM, but the appropriate concentration and molecular weight needed to minimize chronic inflammation within the scaffold remains to be determined.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Materiales Biocompatibles
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Tiramina
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Andamios del Tejido
/
Fascia
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Ácido Hialurónico
Tipo de estudio:
Evaluation_studies
/
Prognostic_studies
Límite:
Adolescent
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Adult
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Animals
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Humans
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Male
/
Middle aged
Idioma:
En
Revista:
J Mater Sci Mater Med
Asunto de la revista:
ENGENHARIA BIOMEDICA
Año:
2011
Tipo del documento:
Article
País de afiliación:
Estados Unidos