Transcriptional landscape of bone marrow-derived very small embryonic-like stem cells during hypoxia.
Respir Res
; 12: 63, 2011 May 10.
Article
en En
| MEDLINE
| ID: mdl-21569252
ABSTRACT
BACKGROUND:
Hypoxia is a ubiquitous feature of many lung diseases and elicits cell-specific responses. While the effects of hypoxia on stem cells have been examined under in vitro conditions, the consequences of in vivo oxygen deprivation have not been studied.METHODS:
We investigated the effects of in vivo hypoxia on a recently characterized population of pluripotent stem cells known as very small embryonic-like stem cells (VSELs) by whole-genome expression profiling and measuring peripheral blood stem cell chemokine levels.RESULTS:
We found that exposure to hypoxia in mice mobilized VSELs from the bone marrow to peripheral blood, and induced a distinct genome-wide transcriptional signature. Applying a computationally-intensive methodology, we identified a hypoxia-induced gene interaction network that was functionally enriched in a diverse array of programs including organ-specific development, stress response, and wound repair. Topographic analysis of the network highlighted a number of densely connected hubs that may represent key controllers of stem cell response during hypoxia and, therefore, serve as putative targets for altering the pathophysiologic consequences of hypoxic burden.CONCLUSIONS:
A brief exposure to hypoxia recruits pluripotent stem cells to the peripheral circulation and actives diverse transcriptional programs that are orchestrated by a selective number of key genes.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
ARN Mensajero
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Células de la Médula Ósea
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Perfilación de la Expresión Génica
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Células Madre Pluripotentes
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Forma de la Célula
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Células Madre Embrionarias
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Hipoxia
Límite:
Animals
Idioma:
En
Revista:
Respir Res
Año:
2011
Tipo del documento:
Article
País de afiliación:
Estados Unidos