A monoclonal antibody against PDGF B-chain inhibits PDGF-induced DNA synthesis in C3H fibroblasts and prevents binding of PDGF to its receptor.
Biochim Biophys Acta
; 1054(2): 246-9, 1990 Sep 01.
Article
en En
| MEDLINE
| ID: mdl-2169313
ABSTRACT
A monoclonal antibody (MAb 6D11) against platelet-derived growth factor (PDGF) was studied. We found that the MAb 6D11 in concentrations equimolar to PDGF blocked the [3H]thymidine incorporation in C3H/10T1/2 C18 fibroblasts stimulated by PDGF B-B and PDGF A-B. This inhibition was overcome by high doses of PDGF. The [3H]thymidine incorporation stimulated by other growth factors (aFGF, bFGF and bombesin) was not inhibited by the antibody. The MAb 6D11 blocked receptor binding of PDGF B-B, but not PDGF A-A. These findings suggest that the MAb 6D11 abolishes PDGF-induced DNA synthesis by blocking PDGF receptor binding. In this communication we demonstrate an isoform-specific monoclonal antibody against PDGF.
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Bases de datos:
MEDLINE
Asunto principal:
ADN
/
Factor de Crecimiento Derivado de Plaquetas
/
Receptores de Superficie Celular
/
Anticuerpos Monoclonales
Límite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
1990
Tipo del documento:
Article
País de afiliación:
Noruega