Evidence for extensive non-endocytotic translocation of peptide nucleic acids across mammalian plasma membranes.
Curr Drug Deliv
; 8(5): 526-33, 2011 Sep.
Article
en En
| MEDLINE
| ID: mdl-21696351
ABSTRACT
The ability of peptide nucleic acids (PNA) to enter and to cross filter-grown MDCK, HEK and CHO cells was studied by means of a protocol based on capillary electrophoresis combined with laser-induced fluorescence detection. The used approach avoided possible errors encountered in protocols based on confocal laserscanning microscopy and FACS analysis. In contradiction to the commonly anticipated unability of PNA to cross biomembranes, extensive translocation of unmodified PNA into and across the investigated cell types was found. The transport mode comprised a variety of energy dependent and -independent as well as temperature sensitive mechanisms being probably destined to natural substrates and hijacked by PNA. The presented results suggest active as well as passive export mechanisms rather than poor penetration into cells to be responsible for the only weak biological activity of unmodified PNA.
Buscar en Google
Bases de datos:
MEDLINE
Asunto principal:
Membrana Celular
/
Ácidos Nucleicos de Péptidos
/
Endocitosis
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Curr Drug Deliv
Asunto de la revista:
FARMACIA
/
FARMACOLOGIA
/
TERAPIA POR MEDICAMENTOS
Año:
2011
Tipo del documento:
Article
País de afiliación:
Alemania