Co-expression of multiple target proteins in plants from a tobacco mosaic virus vector using a combination of homologous and heterologous subgenomic promoters.
Arch Virol
; 156(11): 2057-61, 2011 Nov.
Article
en En
| MEDLINE
| ID: mdl-21779909
ABSTRACT
To co-express multiple target proteins, we engineered a single-component chimeric tobacco mosaic virus (TMV)-based vector containing homologous and heterologous capsid protein subgenomic RNA promoters. Delivery of this vector into Nicotiana benthamiana plants via agroinfiltration resulted in co-expression of two reporter genes within a single cell. Furthermore, co-expression of a host-specific antisense RNA or a silencing suppressor protein from this vector augmented the accumulation of green fluorescent protein or a vaccine antigen, hemagglutinin from avian influenza virus A/Vietnam/1194/04. These findings suggest that this chimeric vector utilizing the homologous and heterologous subgenomic TMV promoters has a potential for high-level production of multiple therapeutic proteins including monoclonal antibodies.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Virus del Mosaico del Tabaco
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Proteínas Recombinantes
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Ingeniería Genética
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Expresión Génica
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Regiones Promotoras Genéticas
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Vectores Genéticos
Idioma:
En
Revista:
Arch Virol
Año:
2011
Tipo del documento:
Article
País de afiliación:
Estados Unidos