Identification of Epstein-Barr virus-infected CD27+ memory B-cells in liver or stem cell transplant patients.
J Gen Virol
; 92(Pt 11): 2590-2595, 2011 Nov.
Article
en En
| MEDLINE
| ID: mdl-21813707
ABSTRACT
To analyse the phenotype of Epstein-Barr virus (EBV)-infected lymphocytes in EBV-associated infections, cells from eight haematopoietic stem cell/liver transplantation recipients with elevated EBV viral loads were examined by a novel quantitative assay designed to identify EBV-infected cells by using a flow cytometric detection of fluorescent in situ hybridization (FISH) assay. By this assay, 0.05-0.78% of peripheral blood lymphocytes tested positive for EBV, and the EBV-infected cells were CD20+ B-cells in all eight patients. Of the CD20+ EBV-infected lymphocytes, 48-83% of cells tested IgD positive and 49-100% of cells tested CD27 positive. Additionally, the number of EBV-infected cells assayed by using FISH was significantly correlated with the EBV-DNA load, as determined by real-time PCR (r2 â= 0.88, P < 0.0001). The FISH assay enabled us to characterize EBV-infected cells and perform a quantitative analysis in patients with EBV infection after stem cell/liver transplantation.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Trasplante
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Linfocitos B
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Miembro 7 de la Superfamilia de Receptores de Factores de Necrosis Tumoral
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Herpesvirus Humano 4
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Infecciones por Virus de Epstein-Barr
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Trasplante de Células Madre
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Hígado
Tipo de estudio:
Diagnostic_studies
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Prognostic_studies
Límite:
Child
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Child, preschool
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Female
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Humans
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Infant
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Male
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Middle aged
Idioma:
En
Revista:
J Gen Virol
Año:
2011
Tipo del documento:
Article
País de afiliación:
Japón