Unfertilized Xenopus eggs die by Bad-dependent apoptosis under the control of Cdk1 and JNK.
PLoS One
; 6(8): e23672, 2011.
Article
en En
| MEDLINE
| ID: mdl-21858202
ABSTRACT
Ovulated eggs possess maternal apoptotic execution machinery that is inhibited for a limited time. The fertilized eggs switch off this time bomb whereas aged unfertilized eggs and parthenogenetically activated eggs fail to stop the timer and die. To investigate the nature of the molecular clock that triggers the egg decision of committing suicide, we introduce here Xenopus eggs as an in vivo system for studying the death of unfertilized eggs. We report that after ovulation, a number of eggs remains in the female body where they die by apoptosis. Similarly, ovulated unfertilized eggs recovered in the external medium die within 72 h. We showed that the death process depends on both cytochrome c release and caspase activation. The apoptotic machinery is turned on during meiotic maturation, before fertilization. The death pathway is independent of ERK but relies on activating Bad phosphorylation through the control of both kinases Cdk1 and JNK. In conclusion, the default fate of an unfertilized Xenopus egg is to die by a mitochondrial dependent apoptosis activated during meiotic maturation.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Óvulo
/
Proteína Quinasa CDC2
/
Apoptosis
/
Proteínas de Xenopus
/
Proteínas Quinasas JNK Activadas por Mitógenos
/
Proteína Letal Asociada a bcl
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
PLoS One
Asunto de la revista:
CIENCIA
/
MEDICINA
Año:
2011
Tipo del documento:
Article
País de afiliación:
Francia