Inhibition of type I interferon production via suppressing IKK-gamma expression: a new strategy for counteracting host antiviral defense by influenza A viruses?
J Proteome Res
; 11(1): 217-23, 2012 Jan 01.
Article
en En
| MEDLINE
| ID: mdl-22054014
ABSTRACT
Blockage of the induction of type I interferons (IFNs) is essential for the success of influenza virus proliferation in host cells. Several molecular mechanisms by which influenza viruses inhibit IFN induction have been characterized. Here we report a potentially new strategy influenza viruses employ to inhibit IFN production during viral infection. Through a two-dimensional gel electrophoresis based proteomic approach, we found that the expression of IκB kinase-gamma (IKKγ) was suppressed by influenza A virus infection in human lung epithelial A549 cells. Silencing of cellular IKKγ by small interfering RNA led to enhanced replication of influenza viruses. Concomitantly, overexpression of IKKγ resulted in increased production of IFNα/ß, whereas influenza virus infection completely eliminated the IKKγ-overexpression-induced production of IFNα/ß. Our results suggest that IKKγ and influenza virus are mutually inhibitory, and influenza viruses may inhibit IFN production through suppressing the expression of IKKγ during viral infection.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Virus de la Influenza A
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Interferón beta
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Interferón-alfa
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Quinasa I-kappa B
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Gripe Humana
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Evasión Inmune
Límite:
Animals
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Humans
Idioma:
En
Revista:
J Proteome Res
Asunto de la revista:
BIOQUIMICA
Año:
2012
Tipo del documento:
Article
País de afiliación:
Estados Unidos