[Optimization of ultrasonic-assisted extraction of total flavonoids from leaves of the Artocarpus heterophyllus by response surface methodology].

ABSTRACT

OBJECTIVE: To investigate the ultrasonic-assisted extract on of total flavonoids from leaves of the Artocarpus heterophyllus. METHODS: Investigated the effects of ethanol concentration, extraction time, and liquid-solid ratio on flavonoids yield. A 17-run response surface design involving three factors at three levels was generated by the Design-Expert software and experimental data obtained were subjected to quadratic regression analysis to create a mathematical model describing flavonoids extraction. RESULTS: The optimum ultrasonic assisted extraction conditions were ethanol volume fraction 69.4% and liquid-solid ratio of 22.61 for 32 min. Under these optimized conditions, the yield of flavonoids was 7.55 mg/g. CONCLUSION: The Box-Behnken design and response surface analysis can well optimize the ultrasonic-assisted extraction of total flavonoids from Artocarpus heterophyllus.