Identification of forensically important flesh flies based on a shorter fragment of the cytochrome oxidase subunit I gene in China.
Med Vet Entomol
; 26(3): 307-13, 2012 Sep.
Article
en En
| MEDLINE
| ID: mdl-22150605
ABSTRACT
With the development of molecular identification, there has been a great deal of discussion about the feature of the mitochondrial DNA (mtDNA) fragments. Although longer fragments may minimize stochastic variation across taxa and be more likely to reflect broader patterns of nucleotide divergence, shorter fragments have many advantages, such as quick, easy and economical. Extensive application of long mtDNA segments for species identification cannot always be achieved as a result of constraints in time and money. In the present study, a molecular identification method involving the sequencing of a 272-bp 'barcode' fragment of the mitochondrial cytochrome oxidase subunit I (COI) gene from 55 specimens, representing 7 Chinese sarcophagid species from varying populations, was evaluated. Phylogenetic analysis of the sequenced segments showed that all sarcophagid specimens were properly assigned into seven species, which indicated the possibility of separation congeneric species with the short fragments. The results of this research will be instrumental for the implementation of the Chinese Sarcophagidae database.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Ciencias Forenses
/
Entomología
/
Sarcofágidos
/
Código de Barras del ADN Taxonómico
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Límite:
Animals
País/Región como asunto:
Asia
Idioma:
En
Revista:
Med Vet Entomol
Asunto de la revista:
BIOLOGIA
/
MEDICINA VETERINARIA
Año:
2012
Tipo del documento:
Article
País de afiliación:
China