Protein B5 is required on extracellular enveloped vaccinia virus for repulsion of superinfecting virions.
J Gen Virol
; 93(Pt 9): 1876-1886, 2012 Sep.
Article
en En
| MEDLINE
| ID: mdl-22622330
ABSTRACT
Vaccinia virus (VACV) spreads across cell monolayers fourfold faster than predicted from its replication kinetics. Early after infection, infected cells repulse some superinfecting extracellular enveloped virus (EEV) particles by the formation of actin tails from the cell surface, thereby causing accelerated spread to uninfected cells. This strategy requires the expression of two viral proteins, A33 and A36, on the surface of infected cells and upon contact with EEV this complex induces actin polymerization. Here we have studied this phenomenon further and investigated whether A33 and A36 expression in cell lines causes an increase in VACV plaque size, whether these proteins are able to block superinfection by EEV, and which protein(s) on the EEV surface are required to initiate the formation of actin tails from infected cells. Data presented show that VACV plaque size was not increased by expression of A33 and A36, and these proteins did not block entry of the majority of EEV binding to these cells. In contrast, expression of proteins A56 and K2 inhibited entry of both EEV and intracellular mature virus. Lastly, VACV protein B5 was required on EEV to induce the formation of actin tails at the surface of cells expressing A33 and A36, and B5 short consensus repeat 4 is critical for this induction.
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Bases de datos:
MEDLINE
Asunto principal:
Vaccinia
/
Virus Vaccinia
/
Virión
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Sobreinfección
/
Membrana Celular
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Proteínas de la Matriz Viral
Límite:
Humans
Idioma:
En
Revista:
J Gen Virol
Año:
2012
Tipo del documento:
Article
País de afiliación:
Reino Unido