The let-7-Imp axis regulates ageing of the Drosophila testis stem-cell niche.
Nature
; 485(7400): 605-10, 2012 May 23.
Article
en En
| MEDLINE
| ID: mdl-22660319
ABSTRACT
Adult stem cells support tissue homeostasis and repair throughout the life of an individual. During ageing, numerous intrinsic and extrinsic changes occur that result in altered stem-cell behaviour and reduced tissue maintenance and regeneration. In the Drosophila testis, ageing results in a marked decrease in the self-renewal factor Unpaired (Upd), leading to a concomitant loss of germline stem cells. Here we demonstrate that IGF-II messenger RNA binding protein (Imp) counteracts endogenous small interfering RNAs to stabilize upd (also known as os) RNA. However, similar to upd, Imp expression decreases in the hub cells of older males, which is due to the targeting of Imp by the heterochronic microRNA let-7. In the absence of Imp, upd mRNA therefore becomes unprotected and susceptible to degradation. Understanding the mechanistic basis for ageing-related changes in stem-cell behaviour will lead to the development of strategies to treat age-onset diseases and facilitate stem-cell-based therapies in older individuals.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Testículo
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Proteínas de Unión al ARN
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Senescencia Celular
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Proteínas de Drosophila
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MicroARNs
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Drosophila melanogaster
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Nicho de Células Madre
Límite:
Animals
Idioma:
En
Revista:
Nature
Año:
2012
Tipo del documento:
Article
País de afiliación:
Estados Unidos