Specific detection by the polymerase chain reaction of potentially allergenic salmonid fish residues in processed foods.
Biosci Biotechnol Biochem
; 76(5): 980-5, 2012.
Article
en En
| MEDLINE
| ID: mdl-22738970
ABSTRACT
Salmonid fish is one of the allergenic items that are recommended to be labeled in the Japanese allergen-labeling system. This study develops a salmonid-specific polymerase chain reaction (PCR) method. A new primer pair, SKE-F/SKE-R, was designed to specifically detect the salmonid fish gene encoding mitochondrial DNA cytochrome b. Genomic DNAs extracted from 58 kinds of seafood and 11 kinds of processed food were individually subjected to PCR by using the primer pair, and a salmonid-specific fragment of 212 bp was only amplified in the salmonid samples and salmonid-containing processed foods. The detection limit of the PCR method was as low as 0.02 fg/µL of salmonid fish DNA (corresponding to 10 copies). There is no ELISA method for salmonid fish, making our PCR method the only reliable measure for detecting salmonid fish in processed foods.
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Bases de datos:
MEDLINE
Asunto principal:
Salmonidae
/
ADN Mitocondrial
/
Alérgenos
/
Reacción en Cadena de la Polimerasa
/
Alimentos Marinos
/
Citocromos b
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
/
Humans
País/Región como asunto:
Asia
Idioma:
En
Revista:
Biosci Biotechnol Biochem
Asunto de la revista:
BIOQUIMICA
/
BIOTECNOLOGIA
Año:
2012
Tipo del documento:
Article
País de afiliación:
Japón