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Comprehensive identification of erythrocyte membrane protein deficiency by 2D gel electrophoresis based proteomic analysis in hereditary elliptocytosis and spherocytosis.
Demiralp, Duygu Ozel; Peker, Selen; Turgut, Burhan; Akar, Nejat.
Afiliación
  • Demiralp DO; Ankara University Biotechnology Institute, Ankara, Turkey. ozel@medicine.ankara.edu.tr
Proteomics Clin Appl ; 6(7-8): 403-11, 2012 Aug.
Article en En | MEDLINE | ID: mdl-22807418
ABSTRACT

PURPOSE:

Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (1DE) may reveal qualitative or quantitative defects in red blood cell (RBC) membrane proteins, two-dimensional gel electrophoresis (2DE) can be used for determination of the protein changes caused by the disease process in a relatively high-throughput manner, because it permits an analysis of thousands of modified or unmodified proteins simultaneously. The principal aim of this study was to compare hereditary elliptocytosis (HE), hereditary spherocytosis (HS), and control RBC membrane protein profiles and identify proteins as a clinical marker by the sensitive methods. EXPERIMENTAL

DESIGN:

RBC membrane proteins of HE, HS, and control groups were compared by 2DE and matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) MS analysis was obtained using peptide mass fingerprint for protein identification.

RESULTS:

Twenty proteins were identified with peptide mass fingerprint analysis and different expression levels were found in HE, HS, and controls for some proteins that includes three biomarker proteins (ankyrin, spectrin, band 3) that may have prognostic or predictive importance. CONCLUSIONS AND CLINICAL RELEVANCE 2DE of RBC proteins is a potentially valuable method for studying heritable disorders such as HE and HS. By identifying a deficiency in membrane proteins associated with the RBC cytoskeleton, the diagnosis of HE and HS can be confirmed.
Asunto(s)

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Esferocitosis Hereditaria / Electroforesis en Gel Bidimensional / Proteómica / Eliptocitosis Hereditaria / Membrana Eritrocítica / Proteínas de la Membrana Tipo de estudio: Diagnostic_studies / Observational_studies / Qualitative_research / Risk_factors_studies Límite: Humans Idioma: En Revista: Proteomics Clin Appl Asunto de la revista: BIOQUIMICA Año: 2012 Tipo del documento: Article País de afiliación: Turquía

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Esferocitosis Hereditaria / Electroforesis en Gel Bidimensional / Proteómica / Eliptocitosis Hereditaria / Membrana Eritrocítica / Proteínas de la Membrana Tipo de estudio: Diagnostic_studies / Observational_studies / Qualitative_research / Risk_factors_studies Límite: Humans Idioma: En Revista: Proteomics Clin Appl Asunto de la revista: BIOQUIMICA Año: 2012 Tipo del documento: Article País de afiliación: Turquía