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Bioinformatic analysis of barcoded cDNA libraries for small RNA profiling by next-generation sequencing.
Farazi, Thalia A; Brown, Miguel; Morozov, Pavel; Ten Hoeve, Jelle J; Ben-Dov, Iddo Z; Hovestadt, Volker; Hafner, Markus; Renwick, Neil; Mihailovic, Aleksandra; Wessels, Lodewyk F A; Tuschl, Thomas.
Afiliación
  • Farazi TA; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, NY 10065, USA.
Methods ; 58(2): 171-87, 2012 Oct.
Article en En | MEDLINE | ID: mdl-22836126
The characterization of post-transcriptional gene regulation by small regulatory RNAs of 20-30 nt length, particularly miRNAs and piRNAs, has become a major focus of research in recent years. A prerequisite for the characterization of small RNAs is their identification and quantification across different developmental stages, normal and diseased tissues, as well as model cell lines. Here we present a step-by-step protocol for the bioinformatic analysis of barcoded cDNA libraries for small RNA profiling generated by Illumina sequencing, thereby facilitating miRNA and other small RNA profiling of large sample collections.
Asunto(s)

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Manejo de Especímenes / Biblioteca de Genes / MicroARNs / Secuenciación de Nucleótidos de Alto Rendimiento Límite: Humans Idioma: En Revista: Methods Asunto de la revista: BIOQUIMICA Año: 2012 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Manejo de Especímenes / Biblioteca de Genes / MicroARNs / Secuenciación de Nucleótidos de Alto Rendimiento Límite: Humans Idioma: En Revista: Methods Asunto de la revista: BIOQUIMICA Año: 2012 Tipo del documento: Article País de afiliación: Estados Unidos