Tracking chromatid segregation to identify human cardiac stem cells that regenerate extensively the infarcted myocardium.
Circ Res
; 111(7): 894-906, 2012 Sep 14.
Article
en En
| MEDLINE
| ID: mdl-22851539
ABSTRACT
RATIONALE According to the immortal DNA strand hypothesis, dividing stem cells selectively segregate chromosomes carrying the old template DNA, opposing accumulation of mutations resulting from nonrepaired replication errors and attenuating telomere shortening. OBJECTIVE:
Based on the premise of the immortal DNA strand hypothesis, we propose that stem cells retaining the old DNA would represent the most powerful cells for myocardial regeneration. METHODS ANDRESULTS:
Division of human cardiac stem cells (hCSCs) by nonrandom and random segregation of chromatids was documented by clonal assay of bromodeoxyuridine-tagged hCSCs. Additionally, their growth properties were determined by a series of in vitro and in vivo studies. We report that a small class of hCSCs retain during replication the mother DNA and generate 2 daughter cells, which carry the old and new DNA, respectively. hCSCs with immortal DNA form a pool of nonsenescent cells with longer telomeres and higher proliferative capacity. The self-renewal and long-term repopulating ability of these cells was shown in serial-transplantation assays in the infarcted heart; these cells created a chimeric organ, composed of spared rat and regenerated human cardiomyocytes and coronary vessels, leading to a remarkable restoration of cardiac structure and function. The documentation that hCSCs divide by asymmetrical and symmetrical chromatid segregation supports the view that the human heart is a self-renewing organ regulated by a compartment of resident hCSCs.CONCLUSIONS:
The impressive recovery in ventricular hemodynamics and anatomy mediated by clonal hCSCs carrying the "mother" DNA underscores the clinical relevance of this stem cell class for the management of heart failure in humans.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Regeneración
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Células Madre
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Cromátides
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Segregación Cromosómica
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Trasplante de Células Madre
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Corazón
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Infarto del Miocardio
/
Miocardio
Límite:
Adolescent
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Adult
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Aged
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Aged80
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Animals
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Child
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Child, preschool
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Female
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Humans
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Infant
Idioma:
En
Revista:
Circ Res
Año:
2012
Tipo del documento:
Article
País de afiliación:
Estados Unidos