Single-stranded microRNA mimics.
RNA
; 18(10): 1796-804, 2012 Oct.
Article
en En
| MEDLINE
| ID: mdl-22912485
ABSTRACT
miRNAs are â¼22-nt RNAs that bind to the Argonaute family of proteins and have important regulatory roles in plants and animals. Here, we show that miRNAs exhibit targeting activity in cells when delivered as single strands that are 5'-phosphorylated and that contain 2'-fluoro ribose modifications. Length preferences, chemical modification sensitivity, and genome-wide seed-based targeting all suggest that this activity is Ago-based. Activity could be enhanced by annealing of segmented passenger strands containing non-nucleic acid spacers. Furthermore, screening of randomly generated sequences identified pyrimidine rich 3' cassette sequences that increased single strand activity. These results provide an initial step in the development of single-stranded miRNA mimics for therapeutic use.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
ADN de Cadena Simple
/
Imitación Molecular
/
MicroARNs
Tipo de estudio:
Evaluation_studies
Límite:
Humans
Idioma:
En
Revista:
RNA
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2012
Tipo del documento:
Article
País de afiliación:
Estados Unidos