Conjugation of Y. pestis F1-antigen to gold nanoparticles improves immunogenicity.
Vaccine
; 30(48): 6777-82, 2012 Nov 06.
Article
en En
| MEDLINE
| ID: mdl-23000121
ABSTRACT
The efficacy of 15 nm gold nanoparticles (AuNP) coated with Yersinia pestis F1-antigen, as an immunogen in mice, has been assessed. The nanoparticles were decorated with F1-antigen using N-hydroxysuccinimide and N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride coupling chemistry. Mice given AuNP-F1 in alhydrogel generated the greatest IgG antibody response to F1-antigen when compared with mice given AuNP-F1 in PBS or given unconjugated F1-antigen in PBS or alhydrogel. Compared with unconjugated F1-antigen, the IgG2a response was enhanced in mice dosed with AuNP-F1 in PBS (p<0.05) but not in mice immunised with AuNP-F1 in alhydrogel. All treatment groups developed a memory response to F1-antigen, the polarity of which was inflenced by formulation in alhydrogel. The sera raised against F1-antigen coupled to AuNPs was able to competitively bind to rF1-antigen, displacing protective macaque sera.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
Portadores de Fármacos
/
Vacuna contra la Peste
/
Adyuvantes Inmunológicos
/
Nanopartículas
/
Oro
Límite:
Animals
Idioma:
En
Revista:
Vaccine
Año:
2012
Tipo del documento:
Article
País de afiliación:
Reino Unido