Visualizing viral assemblies in a nanoscale biosphere.
Lab Chip
; 13(2): 216-9, 2013 Jan 21.
Article
en En
| MEDLINE
| ID: mdl-23208001
ABSTRACT
We present a novel microfluidic platform to examine biological assemblies at high-resolution. We have engineered a functionalized chamber that serves as a "nanoscale biosphere" to capture and maintain rotavirus double-layered particles (DLPs) in a liquid environment. The chamber can be inserted into the column of a transmission electron microscope while being completely isolated from the vacuum system. This configuration allowed us to determine the structure of biological complexes at nanometer-resolution within a self-contained vessel. Images of DLPs were used to calculate the first 3D view of macromolecules in solution. We refer to this new fluidic visualization technology as in situ molecular microscopy.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Rotavirus
/
Técnicas Analíticas Microfluídicas
Idioma:
En
Revista:
Lab Chip
Asunto de la revista:
BIOTECNOLOGIA
/
QUIMICA
Año:
2013
Tipo del documento:
Article
País de afiliación:
Estados Unidos