Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny.
PLoS One
; 7(12): e51361, 2012.
Article
en En
| MEDLINE
| ID: mdl-23300543
ABSTRACT
The development of a megakaryocyte lineage specific Cre deleter, using the Pf4 (CXCL4) promoter (Pf4-Cre), was a significant step forward in the specific analysis of platelet and megakaryocyte cell biology. However, in the present study we have employed a sensitive reporter-based approach to demonstrate that Pf4-Cre also recombines in a significant proportion of both fetal liver and bone marrow hematopoietic stem cells (HSCs), including the most primitive fraction containing the long-term repopulating HSCs. Consequently, we demonstrate that Pf4-Cre activity is not megakaryocyte lineage-specific but extends to other myeloid and lymphoid lineages at significant levels between 15-60%. Finally, we show for the first time that Pf4 transcripts are present in adult HSCs and primitive hematopoietic progenitor cells. These results have fundamental implications for the use of the Pf4-Cre mouse model and for our understanding of a possible role for Pf4 in the development of the hematopoietic lineage.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Factor Plaquetario 4
/
Células de la Médula Ósea
/
Células Madre Hematopoyéticas
/
Megacariocitos
/
Linaje de la Célula
/
Integrasas
/
Feto
/
Hígado
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
PLoS One
Asunto de la revista:
CIENCIA
/
MEDICINA
Año:
2012
Tipo del documento:
Article
País de afiliación:
Reino Unido