Gobichelin A and B: Mixed-Ligand Siderophores Discovered Using Proteomics.
Medchemcomm
; 4(1): 233-238, 2013 Jan 01.
Article
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| MEDLINE
| ID: mdl-23336063
ABSTRACT
"Omic" strategies have been increasingly applied to natural product discovery processes, with (meta-)genome sequencing and mining implemented in many laboratories to date. Using the proteomics-based discovery platform called PrISM (Proteomic Investigation of Secondary Metabolism), we discovered two new siderophores gobichelin A and B from Streptomyces sp. NRRL F-4415, a strain without a sequenced genome. Using the proteomics information as a guide, the 37 kb gene cluster responsible for production of gobichelins was sequenced and its 20 open reading frames interpreted into a biosynthetic scheme. This led to the targeted detection and structure elucidation of the new compounds produced by nonribosomal peptide (NRP) synthesis.
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Medchemcomm
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2013
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