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Production of a human neutralizing monoclonal antibody and its crystal structure in complex with ectodomain 3 of the interleukin-13 receptor α1.
Biochem J ; 451(2): 165-75, 2013 Apr 15.
Article en En | MEDLINE | ID: mdl-23384096
ABSTRACT
Gene deletion studies in mice have revealed critical roles for IL (interleukin)-4 and -13 in asthma development, with the latter controlling lung airways resistance and mucus secretion. We have now developed human neutralizing monoclonal antibodies against human IL-13Rα1 (IL-13 receptor α1) subunit that prevent activation of the receptor complex by both IL-4 and IL-13. We describe the crystal structures of the Fab fragment of antibody 10G5H6 alone and in complex with D3 (ectodomain 3) of IL-13Rα1. Although the structure showed significant domain swapping within a D3 dimer, we showed that Arg(230), Phe(233), Tyr(250), Gln(252) and Leu(293) in each D3 monomer and Ser(32), Asn(102) and Trp(103) in 10G5H6 Fab are the key interacting residues at the interface of the 10G5H6 Fab-D3 complex. One of the most striking contacts is the insertion of the ligand-contacting residue Leu(293) of D3 into a deep pocket on the surface of 10G5H6 Fab, and this appears to be a central determinant of the high binding affinity and neutralizing activity of the antibody.
Asunto(s)

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Subunidad alfa1 del Receptor de Interleucina-13 / Anticuerpos Neutralizantes / Anticuerpos Monoclonales / Epítopos Límite: Animals / Humans Idioma: En Revista: Biochem J Año: 2013 Tipo del documento: Article País de afiliación: Australia

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Subunidad alfa1 del Receptor de Interleucina-13 / Anticuerpos Neutralizantes / Anticuerpos Monoclonales / Epítopos Límite: Animals / Humans Idioma: En Revista: Biochem J Año: 2013 Tipo del documento: Article País de afiliación: Australia