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The PrP(C) C1 fragment derived from the ovine A136R154R171PRNP allele is highly abundant in sheep brain and inhibits fibrillisation of full-length PrP(C) protein in vitro.
Campbell, Lauren; Gill, Andrew C; McGovern, Gillian; Jalland, Clara M O; Hopkins, John; Tranulis, Michael A; Hunter, Nora; Goldmann, Wilfred.
Afiliación
  • Campbell L; The Roslin Institute, University of Edinburgh, Easter Bush, Midlothian, Scotland, UK. Lauren.campbell@roslin.ed.ac.uk
Biochim Biophys Acta ; 1832(6): 826-36, 2013 Jun.
Article en En | MEDLINE | ID: mdl-23474307
Expression of the cellular prion protein (PrP(C)) is crucial for the development of prion diseases. Resistance to prion diseases can result from reduced availability of the prion protein or from amino acid changes in the prion protein sequence. We propose here that increased production of a natural PrP α-cleavage fragment, C1, is also associated with resistance to disease. We show, in brain tissue, that ARR homozygous sheep, associated with resistance to disease, produced PrP(C) comprised of 25% more C1 fragment than PrP(C) from the disease-susceptible ARQ homozygous and highly susceptible VRQ homozygous animals. Only the C1 fragment derived from the ARR allele inhibits in-vitro fibrillisation of other allelic PrP(C) variants. We propose that the increased α-cleavage of ovine ARR PrP(C) contributes to a dominant negative effect of this polymorphism on disease susceptibility. Furthermore, the significant reduction in PrP(C) ß-cleavage product C2 in sheep of the ARR/ARR genotype compared to ARQ/ARQ and VRQ/VRQ genotypes, may add to the complexity of genetic determinants of prion disease susceptibility.
Asunto(s)

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Péptidos / Encéfalo / Proteínas PrPC / Alelos / Resistencia a la Enfermedad / Homocigoto Límite: Animals Idioma: En Revista: Biochim Biophys Acta Año: 2013 Tipo del documento: Article

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Péptidos / Encéfalo / Proteínas PrPC / Alelos / Resistencia a la Enfermedad / Homocigoto Límite: Animals Idioma: En Revista: Biochim Biophys Acta Año: 2013 Tipo del documento: Article