Upregulation of Nrf2 expression by human cytomegalovirus infection protects host cells from oxidative stress.
J Gen Virol
; 94(Pt 7): 1658-1668, 2013 Jul.
Article
en En
| MEDLINE
| ID: mdl-23580430
ABSTRACT
NF-E2 related factor 2 (Nrf2) is a transcription factor that plays a key role(s) in cellular defence against oxidative stress. In this study, we showed that the expression of Nrf2 was upregulated in primary human foreskin fibroblasts (HFFs), following human cytomegalovirus (HCMV/HHV-5) infection. The expression of haem oxygenase-1, a downstream target of Nrf2, was also increased by HCMV infection, and this induction was suppressed in HFFs expressing a small hairpin RNA (shRNA) against Nrf2. The HCMV-mediated increase in Nrf2 expression was abolished when UV-irradiated virus was used or when the activity of casein kinase 2 was inhibited. Host cells infected by HCMV had higher survival rates following oxidative stress induced by buthionine sulfoximine compared with uninfected control cells, but this cell-protective effect was abolished by the use of Nrf2 shRNA. Our results suggest that HCMV-mediated activation of Nrf2 might be beneficial to the virus by increasing the host cell's ability to cope with oxidative stress resulting from viral infection and/or inflammation.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Regulación hacia Arriba
/
Estrés Oxidativo
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Citomegalovirus
/
Factor 2 Relacionado con NF-E2
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Fibroblastos
Límite:
Humans
Idioma:
En
Revista:
J Gen Virol
Año:
2013
Tipo del documento:
Article