Booster responses by oral vaccination with transgenic plants against chicken leucocytozoonosis.
Avian Pathol
; 42(3): 215-20, 2013.
Article
en En
| MEDLINE
| ID: mdl-23607580
ABSTRACT
We developed a transgenic potato (TrP/R7) expressing the recombinant R7 (rR7) antigen for use as an oral vaccine to protect against a chicken protozoan disease, chicken leucocytozoonosis. The TrP/R7 potato was produced by Agrobacterium tumefaciens-mediated transformation and regeneration, and the R7 gene insertion into potato chromosomes was confirmed by genomic polymerase chain reaction and Southern hybridization. rR7 antigen expression in TrP/R7 potato was also confirmed by sandwich enzyme-linked immunosorbent assay and western blotting using an antibody against the second-generation schizont of Leucocytozoon caulleryi. A transgenic potato clone with the highest rR7 antigen expression (3 µg rR7 antigen per gram of fresh-weight potato leaves) was selected, cultivated, and used in oral administration experiments to examine its ability to boost immunity. Chickens were immunized with chicken leucocytozoonosis vaccine "Hokken" by injection, and chickens that developed moderate levels of antibody titres were fed with TrP/R7 leaves. Chickens fed with TrP/R7 leaves showed increased antibody responses. In contrast, chickens fed with non-transgenic potato leaves showed a continuous decrease in antibody titres. Furthermore, chickens fed with TrP/R7 potato leaves showed strong resistance against experimental challenge with L. caulleryi infection. This study demonstrates the use of a plant-based oral vaccine to boost immunity against a protozoan disease.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Enfermedades de las Aves de Corral
/
Infecciones Protozoarias en Animales
/
Vacunas Sintéticas
/
Inmunización Secundaria
/
Plantas Modificadas Genéticamente
/
Haemosporida
Límite:
Animals
Idioma:
En
Revista:
Avian Pathol
Año:
2013
Tipo del documento:
Article
País de afiliación:
Japón