A light sheet based high throughput 3D-imaging flow cytometer for phytoplankton analysis.
Opt Express
; 21(12): 14474-80, 2013 Jun 17.
Article
en En
| MEDLINE
| ID: mdl-23787635
ABSTRACT
This paper reports a light sheet fluorescence imaging flow cytometer for 3D sectioning of phytoplankton. The instrument developed has the inherent advantages of high cell counting throughput and high spatial resolution information derived from flow cytometry and light sheet microscopy. The throughput of the instrument is quantified by the sample volume flow rate of 0.5 µl/min with a spatial resolution as achieved by light sheet microscopy. Preliminary results from 3D morphology of the internal chlorophyll-a structure of two dinoflagellates species show promising application potentials of the method for phytoplankton taxonomy of selected species and species groups.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Fitoplancton
/
Recuento de Células
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Separación Celular
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Imagenología Tridimensional
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Técnicas Analíticas Microfluídicas
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Citometría de Flujo
Idioma:
En
Revista:
Opt Express
Asunto de la revista:
OFTALMOLOGIA
Año:
2013
Tipo del documento:
Article
País de afiliación:
China