Fluorescence detection of cationic amyloid fibrils in human semen.
Bioorg Med Chem Lett
; 23(18): 5199-202, 2013 Sep 15.
Article
en En
| MEDLINE
| ID: mdl-23910594
ABSTRACT
Cationic amyloid fibrils, including the Semen Enhancer of Virus Infection (SEVI), have recently been described in human semen. Simple methods for quantitating these fibrils are needed to improve our understanding of their biological function. We performed high-throughput screening to identify molecules that bind SEVI, and identified a small molecule (8E2), that fluoresced brightly in the presence of SEVI and other cationic fibrils. 8E2 bound SEVI with almost 40-fold greater affinity than thioflavin-T, and could efficiently detect high molecular weight fibrils in human seminal fluid.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Semen
/
Amiloide
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Bioorg Med Chem Lett
Asunto de la revista:
BIOQUIMICA
/
QUIMICA
Año:
2013
Tipo del documento:
Article
País de afiliación:
Estados Unidos