DNA polymerase ß mutations and survival of patients with esophageal squamous cell carcinoma in Linzhou City, China.
Tumour Biol
; 35(1): 553-9, 2014 Jan.
Article
en En
| MEDLINE
| ID: mdl-23959474
ABSTRACT
Linzhou City in northern China has a high incidence of esophageal squamous cell carcinoma (ESCC). This study retrospectively analyzed the data of 231 cases with ESCC collected from 1998 to 2012. Mutations of DNA polymerase ß (polß) gene in the ESCC samples from patients in Linzhou City were examined by amplifying polß cDNA by RT-PCR followed by cloning and sequencing. Mutations in polß were found in 105 cases (45.9%). Nine types of mutations were identified in the polß cDNA; the most common were 177234 nt deletion (11.3%), 462 nt G â T (9.1%), and 648 nt G â C (6.9%). Mutations in polß appeared to be associated with TNM status (P = 0.048). Follow-up data was used for survival analysis. The overall 5-year survival rate of the 231 patients was 37.4%; the rate for patients with wild-type (WT) polß was 41.8%. Compared with the WT polß group, the median survival for patients with specific mutations (177234 nt deletion, 462 nt G â T, or 613 nt A â T) was significantly shorter (all P = 0.000), and the 5-year survival rate decreased to 0%. Patients with the 648 nt G â C mutation had improved survival (P = 0.000) with a 5-year survival rate of 100%. Our results identified nine types of mutations within polß cDNA in ESCC patients with four mutations related to patient survival.
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Bases de datos:
MEDLINE
Asunto principal:
Neoplasias Esofágicas
/
Carcinoma de Células Escamosas
/
ADN Polimerasa beta
/
Mutación
Tipo de estudio:
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Límite:
Adult
/
Aged
/
Female
/
Humans
/
Male
/
Middle aged
País/Región como asunto:
Asia
Idioma:
En
Revista:
Tumour Biol
Asunto de la revista:
NEOPLASIAS
Año:
2014
Tipo del documento:
Article