Adenosina/química; Dominio Catalítico; Metabolismo Energético; ATPasas de Translocación de Protón/química; Adenosina/metabolismo; Adenosina Difosfato/química; Adenosina Difosfato/metabolismo; Adenosina Trifosfato/química; Adenosina Trifosfato/metabolismo; Sitios de Unión; Calorimetría; Proteínas de Unión al ADN/química; Escherichia coli/enzimología; Cinética; Magnesio/química; Magnesio/metabolismo; Nucleótidos/metabolismo; ATPasas de Translocación de Protón/aislamiento & purificación; ATPasas de Translocación de Protón/metabolismo; Termodinámica

2-amino-2-(hydroxymethyl)propane-1,3-diol; ADP; ATP; ATPase; Binding energetics; EDTA; EF1; F(1)-ATPase; F1 sector from Bacillus PS3; F1 sector from Escherichia coli; Heterotropic cooperativity; ITC; Isolated subunit; Isothermal titration calorimetry; K(N1) and K(N2); K(Pf) and K(Pb); K(b); K(d); Metal binding; Mg(II); MgCl2; NMR; S1, S2 and S3; TF1; Tris; adenosine 5'-diphosphate; adenosine 5'-triphosphatase; adenosine 5'-triphosphate; association constants for ATP and Mg·ATP binding to the subunit; binding Gibbs free energy; binding enthalpy; binding entropy; cooperative Gibbs free energy; cooperative enthalpy; cooperative entropy; cooperative heterotropic association constant; equilibrium binding constant; equilibrium dissociation constant; ethylenediaminetetraacetic acid; free magnesium ion; high, medium and low affinity ß-subunit sites in F1, respectively; isothermal titration calorimetry; magnesium chloride; nuclear magnetic resonance; step-wise association constants for the interaction of ATP with Mg(II); ΔG(b); ΔH(b); ΔS(b); Δg; Δh; Δs; ß subunits in the crystal structure of F1 bound to Mg·ADP and Mg·AMPPNP, respectively; ßDP and ßTP; κ