Identification of Haemophilus influenzae and Haemophilus haemolyticus by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
Eur J Clin Microbiol Infect Dis
; 33(2): 279-84, 2014 Feb.
Article
en En
| MEDLINE
| ID: mdl-24019162
ABSTRACT
Generally accepted laboratory methods that have been used for decades do not reliably distinguish between H. influenzae and H. haemolyticus isolates. H. haemolyticus strains are often incorrectly identified as nontypeable Haemophilus influenzae (NTHi). To distinguish H. influenzae from H. haemolyticus we have created a new database on the matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) bio-typer 2 and compared the results with routine determination of Haemophilus (growth requirement for X and V factor), and multilocus sequence typing (MLST). In total we have tested 277 isolates, 244 H. influenzae and 33 H. haemolyticus. Using MLST as the gold standard, the agreement of MALDI-TOF MS was 99.6 %. MALDI-TOF MS allows reliable and rapid discrimination between H. influenzae and H. haemolyticus.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Haemophilus
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Técnicas Bacteriológicas
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Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
Tipo de estudio:
Diagnostic_studies
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Evaluation_studies
/
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Eur J Clin Microbiol Infect Dis
Asunto de la revista:
DOENCAS TRANSMISSIVEIS
/
MICROBIOLOGIA
Año:
2014
Tipo del documento:
Article