A fast fluorescence imaging flow cytometer for phytoplankton analysis.
Opt Express
; 21(20): 23921-6, 2013 Oct 07.
Article
en En
| MEDLINE
| ID: mdl-24104302
ABSTRACT
We report a fast fluorescence imaging flow cytometer for phytoplankton analysis that can achieve a volume flow rate up to 1ml/min. The instrument shows a high immunity to motion blur in image captured with a lateral resolution of 0.75 ± 0.06 µm for a wide size range ~1 µm to ~200 µm. This is made possible by suppressing the out-of-focus light using thin light sheet illumination and image deconvolution, and by precluding the motion-blur with a unique flow configuration. Preliminary results from untreated coastal water samples show the technique has high potential as a practical field instrument for monitoring phytoplankton abundance and species composition.
Texto completo:
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Bases de datos:
MEDLINE
Asunto principal:
Fitoplancton
/
Citometría de Flujo
Idioma:
En
Revista:
Opt Express
Asunto de la revista:
OFTALMOLOGIA
Año:
2013
Tipo del documento:
Article