Role of progesterone in TLR4-MyD88-dependent signaling pathway in pre-eclampsia.
J Huazhong Univ Sci Technolog Med Sci
; 33(5): 730-734, 2013 Oct.
Article
en En
| MEDLINE
| ID: mdl-24142728
ABSTRACT
The role of progesterone in the Toll-like receptor 4 (TLR4)-MyD88-dependent signaling pathway in pre-eclampsia was studied. Peripheral blood mononuclear cells (PBMCs) from pre-eclampsia (PE) patients were subjected to primary culture, and stimulated with different concentrations of progesterone (0, 10(-8), 10(-6), and 10(-4) mol/L). The mRNA expression of TLR4, MyD88 and nuclear factor-kappaB (NF-κB) was detected by using real-time PCR. The Ikappa-B protein expression was detected by using Western blotting. The expression of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the supernatant was determined by using ELISA. With the concentrations of progesterone increasing, the mRNA expression levels of TLR4, MyD88 and NF-κB in 2(-ΔΔCT) value were significantly decreased, and the IkappaB protein expression levels were significantly increased. The TNF-α and IL-6 expression showed a downward trend when the progesterone concentration increased, and there were significant differences among all of the groups (P<0.05). It was suggested that progesterone can inhibit the TLR4-MyD88-dependent signaling pathway in PE significantly and benefit for the pregnancy.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Preeclampsia
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Progesterona
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Leucocitos Mononucleares
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Transducción de Señal
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Receptor Toll-Like 4
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Factor 88 de Diferenciación Mieloide
Límite:
Adult
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Female
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Humans
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Pregnancy
Idioma:
En
Revista:
J Huazhong Univ Sci Technolog Med Sci
Asunto de la revista:
MEDICINA
Año:
2013
Tipo del documento:
Article
País de afiliación:
China