Reactive oxygen species (ROS) protection via cysteine oxidation in the epidermal cornified cell envelope.
Methods Mol Biol
; 1195: 157-69, 2014.
Article
en En
| MEDLINE
| ID: mdl-24281873
ABSTRACT
The outermost layer of our skin functions as a barrier to protect us from physical, chemical, and biological environmental insults. This protective function is mediated by the epidermal cornified cell envelope (CE) which serves both as a mechanical and permeability barrier. Recently we have discovered that the CE constitutes also a first-line antioxidant shield which relies greatly on cysteine residues in CE precursor proteins. Here we describe methods and protocols to study the cysteine-mediated antioxidant function of the CE at the level of the whole organ (the skin), individual cells (keratinocytes), or isolated proteins (SPRR family).
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Especies Reactivas de Oxígeno
/
Cisteína
/
Imagen Molecular
/
Células Epidérmicas
Límite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2014
Tipo del documento:
Article
País de afiliación:
Países Bajos