Cluster of erythrocyte band 3: a potential molecular target of exhaustive exercise-induced dysfunction of erythrocyte deformability.
Can J Physiol Pharmacol
; 91(12): 1127-34, 2013 Dec.
Article
en En
| MEDLINE
| ID: mdl-24289085
ABSTRACT
The aim of this study is to explore the effect of exhaustive exercise on erythrocyte band 3 (SLC4A1; EB3). The association between the alterations of EB3 and red blood cell (RBC) deformability induced by exercise-induced dysfunction has been investigated. Rats were divided among 2 groups (i) control (C), and (ii) exercise exhausted (E). RBC deformability was investigated in the rats in the exhaustive exercise and control groups. Erythrocytes from the control and exercise-exhausted groups were evaluated for the expression of erythrocyte band 3 through immunoblotting and immunofluorescence studies. Exhaustive exercise led to significant increments in the levels of clustering of erythrocyte band 3 along with the conjugation of membrane proteins to form high-molecular-weight complexes (P < 0.05). Under shear stresses, RBC deformability was found to decline significantly in the exhaustive exercise groups compared with the control group. These data suggest that the RBC dysfunction observed during exercise-induced oxidative stress could be associated with alterations in the structure and function of erythrocyte band 3, which in turn leads to dysfunction in the rheological properties of RBCs. These results provide further insight into erythrocyte damage induced by exhaustive exercise.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Condicionamiento Físico Animal
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Proteína 1 de Intercambio de Anión de Eritrocito
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Deformación Eritrocítica
Límite:
Animals
Idioma:
En
Revista:
Can J Physiol Pharmacol
Año:
2013
Tipo del documento:
Article