[Three-dimensional cell cultures. Applications in basic science and biotechnology]. / Trójwymiarowe hodowle komórek--zastosowania w badaniach podstawowych i inzynierii tkankowej.
Postepy Biochem
; 59(3): 305-14, 2013.
Article
en Pl
| MEDLINE
| ID: mdl-24364213
ABSTRACT
The tissue culture technique is widely used in biochemical and molecular studies, offering accessibility to biological material, high reproducibility of results and high throughput format, comparing with organ cultures. However, traditional, two-dimensional cultures (2D cultures) poorly represent the microenvironment of a tissue, and they are gradually replaced with 3D cultures, that enable formation of intercellular contacts, signaling pathways and gene expression characteristic for tissue in vivo. This paper presents the biology of three-dimensional cultures (spheroids), their applications in basic science and biotechnology and methods of spheroids formation.
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Bases de datos:
MEDLINE
Asunto principal:
Esferoides Celulares
/
Técnicas de Cultivo de Célula
Límite:
Animals
/
Humans
Idioma:
Pl
Revista:
Postepy Biochem
Año:
2013
Tipo del documento:
Article
País de afiliación:
Polonia